Roles & Responsibilities:
- Read and understand various in vitro ADME protocols.
- Conduct in vitro stability experiments (liver microsomes, plasma, and other relevant matrices) for new chemical entities.
- Conduct the following in vitro ADME experiments:
- logP, logD, pKa measurements
- CYP induction and inhibition assays
- Permeability determinations (Caco-2, MDCK, etc.) in relevant cell lines
- CYP phenotyping in purified enzyme systems
- Solubility determinations (kinetic, thermodynamic, simulated fluids)
- Read and understand the bioanalysis sections in the study protocols.
- LC-MS/MS and HPLC-UV/PDA method development and validation (partial and complete).
- Develop fit-for-purpose LC-MS/MS methods for routine in vitro and in vivo sample analysis.
- Extract data from the instrument, conduct bioanalytical QC, and share with stakeholders within the DMPK department.
- Experience in handling bioanalytical method development for various matrices (plasma, blood, various tissues, urine, faeces, etc.).
- Develop LC-MS/MS or RF-MS/MS methods for biochemical and cell-based screening of NCEs.
- Conduct biomarker estimations in various matrices.
- General LC-MS/MS and HPLC maintenance and troubleshooting.
